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Micronucleus Test and Comet Assay on Mice Fed Over Five
Generations a Diet Containing Genetically Modified Triticale
Jaszczak K., Kruszewski M., Baranowski A., Parada R., Bartlomiejczyk T., Zimny J., Rosochacki S.
Source: Journal of Animal and Feed Sciences
Year: 2008, Vol: 17, Number: 1, Pages: 100-109
Discipline: ANIMAL SCIENCES
Publication order reference:
K. Jaszczak, Institute of Genetics and Animal Breeding, Polish Academy
of Sciences, Department of Molecular Cytogenetics, Jastrzebiec, 05-552
Wolka Kosowska, Poland
K.Jaszczak@ighz.pl
Abstract: One of the concerns regarding the common use of genetically
modified (GM) feed in animal nutrition is that transgenic sequences
may have a negative effect on the organism or (and) its cells. The
present report assesses the genotoxic potential effect of a diet
containing GM triticale on mice by using micronuclei test and comet
assay. One group of mice (C57 Bl/6J strain) were fed continuously over
five generations a pelleted diet containing 20% of GM triticale
(tolerant to phosphinothricine) grain, while the control group was fed
pellets with 20% conventional triticale grain. Ten 91 -day s-old mice
(five females and five males) were randomly selected from each group
and each generation for micronuclei test in bone marrow and peripheral
blood erythrocytes and the some number of mice was used for comet
assay.
The results obtained did not reveal any statistically
significant differences in the micronuclei frequency nor any other DNA
damage between the control and experimental groups of mice in all five
generations. Thus, it seems evident that the diet containing GM
triticale (with bar transgene) does not induce chromosome damage, nor
has it any effect on the formation of DNA breaks or base lesions. |
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